Journal: FEBS letters

Article Title: Synthetic filaments assembled from C-terminally truncated alpha-synuclein.

doi: 10.1016/s0014-5793(98)01146-6

Figure Lengend Snippet: Fig. 1. K-Synuclein and various C-terminally truncated constructs. A: Diagram of the full-length 140 amino acid protein, showing re- peats and acidic C-terminal region, together with truncated proteins of lengths 130, 120 and 110 amino acids. B: Coomassie brilliant blue stained blot of puri¢ed K-synuclein constructs. C: Immunoblot with antiserum PER4 similar to that shown in B. Lanes 1: Full- length protein; 2: 1^110; 3: 1^120; 4: 1^130.

Article Snippet: Immunoblot analysis was carried out with anti-K-synuclein serum PER4 (diluted 1:1000) [9] and developed using a Vectastain kit (Vector Laboratories, Peterborough, UK).

Techniques: Construct, Staining, Western Blot

Journal: FEBS letters

Article Title: Synthetic filaments assembled from C-terminally truncated alpha-synuclein.

doi: 10.1016/s0014-5793(98)01146-6

Figure Lengend Snippet: Fig. 3. Immunolabelling of synthetic K-synuclein (1^120) ¢laments and ¢laments extracted from diseased brains, labelled with PER4 antiserum (A^E) and labelled with PER1 antiserum (F^I). A^C: K-Synuclein (1^120) ¢laments showing a labelled clump and individ- ual ¢laments. D^E: Filaments extracted from brains with di¡use Lewy body disease (D) or multiple system atrophy (E). F^I: Fila- ments end-labelled with PER1 antiserum, showing K-synuclein (1^ 120) ¢laments (F^H) and a ¢lament from a brain with multiple sys- tem atrophy (I). The 10-nm gold particles attached to the secondary antibody appear as black dots. Scale bar 100 nm.

Article Snippet: Immunoblot analysis was carried out with anti-K-synuclein serum PER4 (diluted 1:1000) [9] and developed using a Vectastain kit (Vector Laboratories, Peterborough, UK).

Techniques: